The Molecular Genetic Purity Test is an advanced DNA-based analytical method used to assess the genetic purity and hybridity of seed samples at the molecular level. The test utilizes co-dominant DNA markers targeting specific genomic regions across different chromosomes of the crop. The analysis involves DNA extraction from seed or leaf samples, followed by PCR amplification and agarose gel electrophoresis. The resulting DNA banding patterns enable clear identification of the female and male parental alleles, confirming their presence or absence in individual hybrid seed samples and thereby providing reliable evidence of genetic purity and hybridity. Compared with conventional field Grow-Out Tests (GOT), molecular genetic purity testing provides a rapid, precise, reliable and laboratory-based assessment, significantly reducing the time and labour required for genetic purity evaluation. This approach supports efficient quality control and helps ensure the genetic identity, purity and authenticity of hybrid seed lots.